Transcriptome analysis for Notch3 target genes identifies Grip2 as a novel regulator of myogenic response in the cerebrovasculature

C Fouillade, C Baron-Menguy… - … , and vascular biology, 2013 - Am Heart Assoc
C Fouillade, C Baron-Menguy, V Domenga-Denier, C Thibault, K Takamiya, R Huganir
Arteriosclerosis, thrombosis, and vascular biology, 2013Am Heart Assoc
Objective—Notch3 is critically important for the structure and myogenic response of distal
arteries, particularly of cerebral arteries. However, signaling pathways acting downstream of
Notch3 remain largely unknown. Methods and Results—Transcriptome analysis using tail
arteries of Notch3-null mice identified a core set of 17 novel Notch3-regulated genes
confirmed in tail or brain arteries. Postnatal deletion of RBP-Jκ in smooth muscle cells
recapitulated the structural, functional, and molecular defects of brain arteries induced by …
Objective
Notch3 is critically important for the structure and myogenic response of distal arteries, particularly of cerebral arteries. However, signaling pathways acting downstream of Notch3 remain largely unknown.
Methods and Results
Transcriptome analysis using tail arteries of Notch3-null mice identified a core set of 17 novel Notch3-regulated genes confirmed in tail or brain arteries. Postnatal deletion of RBP-Jκ in smooth muscle cells recapitulated the structural, functional, and molecular defects of brain arteries induced by Notch3 deficiency. Transient in vivo blockade of the Notch pathway with γ-secretase inhibitors uncovered, in addition to Notch3, 6 immediate responders, including the voltage-gated potassium channel Kv1.5, which opposes to myogenic constriction of brain arteries, and the glutamate receptor–interacting protein 2 (Grip2) with no previously established role in the cerebrovasculature. We identified a vascular smooth muscle cell isoform of Grip2. We showed that Notch3–RBP-Jκ specifically regulates this isoform. Finally, we found that cerebral arteries of Grip2 mutant mice, which express an N-terminally truncated Grip2 protein, exhibited selective attenuation of pressure-induced contraction.
Conclusion
Our data provide insight into how Notch3 signals in the brain arteries, establishing the postnatal requirement of smooth muscle RBP-Jκ in this context. Notch3-regulated transcriptome provides potential for modulating myogenic response in the cerebrovasculature.
Am Heart Assoc