[HTML][HTML] Antigen-specificity of T cell infiltrates in biopsies with T cell–mediated rejection and BK polyomavirus viremia: analysis by next generation sequencing
G Zeng, Y Huang, Z Lyu, D Lesniak… - American Journal of …, 2016 - Elsevier
This study interrogates the antigen-specificity of inflammatory infiltrates in renal biopsies with
BK polyomavirus (BKPyV) viremia (BKPyVM) with or without allograft nephropathy
(BKPyVN). Peripheral blood mononuclear cells (PBMC) from five healthy HLA-A0101
subjects were stimulated by peptides derived from the BKPYV proteome or polymorphic
regions of HLA. Next generation sequencing of the T cell–receptor complementary DNA was
performed on peptide-stimulated PBMC and 23 biopsies with T cell–mediated rejection …
BK polyomavirus (BKPyV) viremia (BKPyVM) with or without allograft nephropathy
(BKPyVN). Peripheral blood mononuclear cells (PBMC) from five healthy HLA-A0101
subjects were stimulated by peptides derived from the BKPYV proteome or polymorphic
regions of HLA. Next generation sequencing of the T cell–receptor complementary DNA was
performed on peptide-stimulated PBMC and 23 biopsies with T cell–mediated rejection …
