Rapid detection and quantification of CMV DNA in urine using LightCycler™-based real-time PCR
AM Kearns, AJL Turner, GJA Eltringham… - Journal of clinical …, 2002 - Elsevier
A real-time quantitative PCR-hybridisation assay was developed for the detection of human
cytomegalovirus DNA in clinical material. The assay is based on a LightCycler (LC) and
provides both rapid results (< 1 h) and quantification over a broad dynamic range (2× 103–
5× 108 CMV DNA copies/ml). Given that the assay showed a 3-fold increase in sensitivity
compared to detection of early antigen fluorescent foci (DEAFF) testing of urine samples, we
investigated the practicality of testing surveillance such specimens from …
cytomegalovirus DNA in clinical material. The assay is based on a LightCycler (LC) and
provides both rapid results (< 1 h) and quantification over a broad dynamic range (2× 103–
5× 108 CMV DNA copies/ml). Given that the assay showed a 3-fold increase in sensitivity
compared to detection of early antigen fluorescent foci (DEAFF) testing of urine samples, we
investigated the practicality of testing surveillance such specimens from …