[HTML][HTML] Vacuolar H+-ATPase binding to microfilaments: regulation in response to phosphatidylinositol 3-kinase activity and detailed characterization of the actin …
SH Chen, MR Bubb, EG Yarmola, J Zuo, J Jiang… - Journal of Biological …, 2004 - ASBMB
Vacuolar H+-ATPase (V-ATPase) binds microfilaments, and that interaction may be
mediated by an actin binding domain in subunit B of the enzyme. To test for possible
physiologic functions of the actin binding activity of V-ATPase, early responses of resorbing
osteoclasts to inhibition of phosphatidylinositol 3-kinase activity by wortmannin and
LY294002 were examined. Rapid co-localization between V-ATPase and F-actin was
demonstrated by immunocytochemistry, and corresponding association between V-ATPase …
mediated by an actin binding domain in subunit B of the enzyme. To test for possible
physiologic functions of the actin binding activity of V-ATPase, early responses of resorbing
osteoclasts to inhibition of phosphatidylinositol 3-kinase activity by wortmannin and
LY294002 were examined. Rapid co-localization between V-ATPase and F-actin was
demonstrated by immunocytochemistry, and corresponding association between V-ATPase …
